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Development of green fluorescent protein-based cAMP indicators for covering a wide range of cAMP concentrations

  • Published:2023 Issue:23 Volume:13 Page:15514-15520
  • ISSN:2046-2069
  • Container-title:RSC Advances
  • language:en
  • Short-container-title:RSC Adv.

Author:

Hiasa Sohei1,Fujimori Takeru1,Aiki Saki2,Ueda Hiroshi3ORCID,Tsuboi Takashi2,Kitaguchi Tetsuya3ORCID

Affiliation:

1. School of Life Science and Technology, Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa, 226-8501, Japan

2. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan

3. Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa, 226-8503, Japan

Abstract

We developed turn-on type green fluorescent cAMP indicators suitable for wide range detection and dual-color imaging.

Funder

Iketani Science and Technology Foundation

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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