Temperature-cycle microscopy reveals single-molecule conformational heterogeneity-Reference-Cited by-同舟云学术

Temperature-cycle microscopy reveals single-molecule conformational heterogeneity

Published:2015 Issue:9 Volume:17 Page:6532-6544
ISSN:1463-9076
Container-title:Physical Chemistry Chemical Physics
language:en
Short-container-title:Phys. Chem. Chem. Phys.

Author:

Yuan Haifeng¹²³⁴,Gaiduk Alexander¹²³⁴,Siekierzycka Joanna R.¹²³⁴,Fujiyoshi Satoru⁵⁶⁷⁸,Matsushita Michio⁵⁶⁷⁸,Nettels Daniel⁹¹⁰¹¹¹²,Schuler Benjamin⁹¹⁰¹¹¹²,Seidel Claus A. M.¹³¹⁴¹⁵¹⁶,Orrit Michel¹²³⁴

Affiliation:

1. Huygens-KamerlingOnnes Laboratory

2. Leiden University

3. Leiden

4. The Netherlands

5. Department of Physics

6. Tokyo Institute of Technology

7. Tokyo

8. Japan

9. Department of Biochemistry

10. University of Zurich

11. Zurich

12. Switzerland

13. Institute for Physical Chemistry

14. Heinrich-Heine-Universität Düsseldorf

15. Düsseldorf

16. Germany

Abstract

Single-molecule temperature-cycle FRET microscopy of polyproline and dsDNA reveals conformational heterogeneity induced dye–dye interaction and its influence on observed FRET.

Funder

European Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CP/C4CP05486E

Reference69 articles.

1. Energy transfer: a spectroscopic ruler.

2. J. R. Lakowicz , Principles of Fluorescence Spectroscopy, Springer, 3rd edn, 2006

3. Polyproline and the "spectroscopic ruler" revisited with single-molecule fluorescence