Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR

Author:

Chavan Dimple1234ORCID,Chen Hui1234,Crum Mary5234,Vu Binh5234,Safari Mohammad5234,Smith Maxwell5234,Vekilov Peter5234,Conrad Jacinta C.5234ORCID,Kourentzi Katerina5234ORCID,Willson Richard C.12345ORCID

Affiliation:

1. Department of Biology and Biochemistry

2. University of Houston

3. Houston

4. USA

5. Department of Chemical and Biomolecular Engineering

Abstract

We have demonstrated a novel immuno-PCR-based diagnostic platform which uses self-assembled ultra-detectable DNA–avidin nanoparticles stabilized with poly(ethylene glycol) for the ultrasensitive detection of protein analytes.

Funder

National Institute of Allergy and Infectious Diseases

National Science Foundation

Cancer Prevention and Research Institute of Texas

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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