Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction-Reference-Cited by-同舟云学术

Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction

Published:2021 Issue:26 Volume:12 Page:9114-9123
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Han Ming-Jie¹²³⁴,He Qing-tao⁵⁶⁷⁸⁹,Yang Mengyi¹⁰¹¹¹²¹³¹⁴,Chen Chao¹²³⁴¹⁵,Yao Yirong¹⁰¹¹¹²¹³¹⁴,Liu Xiaohong¹⁶²¹⁴⁴,Wang Yuchuan¹⁷¹⁸¹⁹⁴,Zhu Zhong-liang¹⁰²⁰⁴,Zhu Kong-kai²¹²²⁹⁴,Qu Changxiu⁵⁶⁷⁸⁹,Yang Fan⁵⁶⁷⁸⁹,Hu Cheng¹⁶²¹⁴⁴,Guo Xuzhen¹⁶²¹⁴⁴,Zhang Dawei¹²³⁴,Chen Chunlai¹⁰¹¹¹²¹³¹⁴,Sun Jin-peng⁵⁶⁷⁸⁹,Wang Jiangyun¹⁶²¹⁴⁴¹⁵^ORCID

Affiliation:

1. Tianjin Institute of Industrial Biotechnology

2. Chinese Academy of Sciences

3. Tianjin

4. China

5. Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology

6. School of Basic Medical Sciences

7. Cheeloo College of Medicine

8. Shandong University

9. Jinan

10. School of Life Sciences

11. Tsinghua-Peking Joint Center for Life Sciences

12. Beijing Advanced Innovation Center for Structural Biology

13. Tsinghua University

14. Beijing

15. University of the Chinese Academy of Sciences (UCAS)

16. Institute of Biophysics

17. Shenzhen Institute of Transfusion Medicine

18. Shenzhen Blood Center

19. Shenzhen

20. University of Science and Technology of China

21. School of Biological Science and Technology

22. University of Jinan

Abstract

A facile bioconjugation reaction for site-specific protein modification was developed for smFRET measurement, which detected the subtle but important conformational change of the β-arrestin/GPCR complex for the first time.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Sanming Project of Medicine in Shenzhen

National Science Fund for Distinguished Young Scholars

Natural Science Foundation of Beijing Municipality

Natural Science Foundation of Shandong Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

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