A fluorescent HTS assay for phosphohydrolases based on nucleoside 5′-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I-Reference-Cited by-同舟云学术

A fluorescent HTS assay for phosphohydrolases based on nucleoside 5′-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I

Published:2016 Issue:20 Volume:14 Page:4595-4604
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Baranowski M. R.¹²³⁴⁵,Nowicka A.¹²³⁴⁵,Jemielity J.⁶⁴⁷⁸^ORCID,Kowalska J.¹²³⁴⁵^ORCID

Affiliation:

1. Division of Biophysics

2. Institute of Experimental Physics

3. Faculty of Physics

4. University of Warsaw

5. 02-089 Warsaw

6. Centre of New Technologies

7. 02-097 Warsaw

8. Poland

Abstract

By using a fluoride-sensitive probe, we harnessed P–F bond cleavage reaction to develop an assay to screen for nucleotide-handling phosphohydrolase inhibitors.

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/OB/C6OB00492J

Reference52 articles.

1. Nucleotide signalling during inflammation

2. Cellular function and molecular structure of ecto-nucleotidases

3. Purinergic Signaling during Inflammation

4. dUTPases, the unexplored family of signalling molecules

5. Mammalian Cyclic Nucleotide Phosphodiesterases: Molecular Mechanisms and Physiological Functions

Cited by 20 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Tf₂O-Mediated P(O)–N Bond Formation of Either P(O)–OH or P(O)–H Reagents with Multitype Amines;The Journal of Organic Chemistry;2024-05-01

2. In situ detection of enzymatic activities by the bioorthogonal reaction-coupled assay using a dialkoxysilylated fluorogenic probe;Chemistry Letters;2024-03-01

3. SO₂F₂-Mediated Fluorination of P(O)–H and P(O)–OH Compounds under Mild Conditions;The Journal of Organic Chemistry;2023-09-01

4. Synthesis and Evaluation of Diguanosine Cap Analogs Modified at the C8-Position by Suzuki–Miyaura Cross-Coupling: Discovery of 7-Methylguanosine-Based Molecular Rotors;The Journal of Organic Chemistry;2023-05-20

5. 5′-Chalcogen-Substituted Nucleoside Pyrophosphate and Phosphate Monoester Analogues: Preparation and Hydrolysis Studies;International Journal of Molecular Sciences;2022-12-08