An “OFF–ON–OFF” fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems-Reference-Cited by-同舟云学术

An “OFF–ON–OFF” fluorescence protein-labeling probe for real-time visualization of the degradation of short-lived proteins in cellular systems

Published:2022 Issue:5 Volume:13 Page:1419-1427
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Reja Shahi Imam¹^ORCID,Hori Yuichiro¹²^ORCID,Kamikawa Takuya¹,Yamasaki Kohei¹,Nishiura Miyako¹,Bull Steven D.³^ORCID,Kikuchi Kazuya¹²^ORCID

Affiliation:

1. Division of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan

2. Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan

3. Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK

Abstract

An “OFF–ON–OFF” fluorescence probe for real-time imaging of the expression (fluorescence ‘OFF’) and degradation (fluorescence ‘ON’) of short lived PYP-tag proteins in cellular systems.

Funder

Tokyo Biochemical Research Foundation

Japan Society for the Promotion of Science

Royal Society of Chemistry

Japan Agency for Medical Research and Development

Core Research for Evolutional Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D1SC06274C

Reference48 articles.

1. Protein degradation and protection against misfolded or damaged proteins

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3. In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

4. Pathology and function of nuclear amyloid

5. Protein aggregation diseases: pathogenicity and therapeutic perspectives

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