‘Traceless’ tracing of proteins – high-affinity trans-splicing directed by a minimal interaction pair
Author:
Affiliation:
1. Institute of Biochemistry, Biocenter, and Cluster of Excellence – Macromolecular Complexes
2. Goethe-University Frankfurt
3. 60438 Frankfurt/M.
4. Germany
Abstract
Using a minimal lock-and-key element the affinity between the intein fragments for N-terminal protein trans-splicing was significantly increased, allowing for site-specific, ‘traceless’ covalent protein labeling in living mammalian cells at nanomolar probe concentrations.
Funder
Deutsche Forschungsgemeinschaft
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2016/SC/C5SC02936H
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