New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis-Reference-Cited by-同舟云学术

New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis

Published:2021 Issue:29 Volume:13 Page:12443-12453
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Safar Wafa¹,Tatar Andra-Sorina²,Leray Aymeric³,Potara Monica²,Liu Qiqian⁴,Edely Mathieu¹,Djaker Nadia⁴^ORCID,Spadavecchia Jolanda⁴,Fu Weiling⁵,Derouich Sarra Gam⁶^ORCID,Felidj Nordin⁶^ORCID,Astilean Simion²^ORCID,Finot Eric³,Lamy de la Chapelle Marc¹⁵⁷^ORCID

Affiliation:

1. IMMM – UMR 6283 CNRS, Le Mans Université, Avenue Olivier Messiaen, 72085 Le Mans, Cedex 9, France

2. Babes-Bolyai University, Institute for Interdisciplinary Research in Bio-Nanosciences and Faculty of Physics, Nanobiophotonics and Laser Microspectroscopy Center, 1 Str. M Kogalniceanu, RO-400084 Cluj-Napoca, Romania

3. Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université de Bourgogne Franche Comté, F-21078 Dijon, France

4. CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques, Université Paris 13, Sorbonne Paris Cité, Bobigny, France

5. Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, China

6. Université de Paris, ITODYS, CNRS, UMR 7086, 15 rue J-A de Baïf, F-75013 Paris, France

7. STAR Babes-Bolyai University Institute, 1 Str. M Kogalniceanu, RO-400084 Cluj-Napoca, Romania

Abstract

Combining SERS and statistical analysis, we demonstrated that an aptamer is a flexible molecule that is blocked in a specific conformation to capture its analyte and we provided evidence for the dynamics of the aptamer/protein interaction.

Funder

European Commission

Agence Nationale de la Recherche

Conseil régional de Bourgogne-Franche-Comté

National Natural Science Foundation of China

Conseil Régional des Pays de la Loire

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2021/NR/D1NR02180J