Label-free Raman mapping of saturated and unsaturated fatty acid uptake, storage, and return toward baseline levels in macrophages

Author:

Sugiyama Takeshi12345,Hobro Alison J.534ORCID,Pavillon Nicolas534ORCID,Umakoshi Takayuki12346ORCID,Verma Prabhat1234ORCID,Smith Nicholas53473

Affiliation:

1. Department of Applied Physics

2. Graduate School of Engineering

3. Osaka University

4. Japan

5. Immunology Frontier Research Center (IFReC)

6. PRESTO

7. Open and Transdisciplinary Research Institute

Abstract

We use Raman imaging and analysis to evaluate the effects of different fatty acids following their uptake in macrophages, evaluating the fatty acid dynamics, endogenous cellular behavior and metabolism.

Funder

Japan Society for the Promotion of Science

Uehara Memorial Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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