Facile preparation of amorphous cobalt phosphate as inorganic carrier for direct separation and immobilization of his-tagged β-glucosidase from cell lysate-Reference-Cited by-同舟云学术

Facile preparation of amorphous cobalt phosphate as inorganic carrier for direct separation and immobilization of his-tagged β-glucosidase from cell lysate

Published:2022 Issue:45 Volume:46 Page:21615-21624
ISSN:1144-0546
Container-title:New Journal of Chemistry
language:en
Short-container-title:New J. Chem.

Author:

Ma Xinnan¹^ORCID,Chen Zhili¹,Han Juan²,Zhou Yang³^ORCID,Mao Yanli⁴^ORCID,Li Chunmei¹^ORCID,Wang Lei¹,Wang Yun¹^ORCID

Affiliation:

1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China

2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China

3. Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu Province, 212012, China

4. Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan 467036, Henan, China

Abstract

The present work was aimed to develop a facile method to fabricate solid support for the separation and immobilization of his-tagged enzymes directly from cell lysate without pre-purification of the enzymes.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Chemistry,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/NJ/D2NJ01148D

Reference45 articles.

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5. Immobilization of β-glucosidase from Aspergillus niger on κ-carrageenan hybrid matrix and its application on the production of reducing sugar from macroalgae cellulosic residue

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