Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels

Author:

Chen Li-Hua1234,Sung Tzu-Cheng1234,Lee Henry Hsin-Chung56748,Higuchi Akon12349ORCID,Su Huan-Chiao1234,Lin Kuan-Ju1234,Huang Yu-Ru1234,Ling Qing-Dong1011124,Kumar S. Suresh13141516,Alarfaj Abdullah A.917181920,Munusamy Murugan A.917181920,Nasu Michiyo21222324,Chen Da-Chung2526274,Hsu Shih-Tien2826274,Chang Yung29303134,Lee Kuei-Fang3233344ORCID,Wang Han-Chow2535364,Umezawa Akihiro21222324

Affiliation:

1. Department of Chemical and Materials Engineering

2. National Central University

3. Taoyuan

4. Taiwan

5. Department of Surgery

6. Hsinchu Cathay General Hospital

7. Hsinchu

8. Graduate Institute of Translational and Interdisciplinary Medicine

9. Department of Botany and Microbiology

10. Cathay Medical Research Institute

11. Cathay General Hospital

12. Taipei

13. Department of Medical Microbiology and Parasitology

14. Universiti Putra Malaysia

15. 43400 Serdang

16. Malaysia

17. College of Science

18. King Saud University

19. Riyadh 11451

20. Saudi Arabia

21. Department of Reproduction

22. National Center for Child Health and Development

23. Tokyo 157-8535

24. Japan

25. Department of Obstetrics and Gynecology

26. Taiwan Landseed Hospital

27. Taoyuan 32405

28. Department of Internal Medicine

29. Department of Chemical Engineering

30. R&D Center for Membrane Technology

31. Chung Yuan Christian University

32. Precision Medical laboratory

33. Lee's OB/GYN Clinic

34. Taipei 106

35. Hungchi Women & Children's Hospital

36. Taoyuan 320

Abstract

Xeno-free culture and cardiomyocyte differentiation of human embryonic stem cells on vitronectin-grafted hydrogels by adjusting surface charge and elasticity.

Funder

Ministry of Science and Technology, Taiwan

Landseed Hospital

Cathay General Hospital

Japan Science and Technology Agency

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

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1. Atelocollagen supports three-dimensional culture of human induced pluripotent stem cells;Molecular Therapy - Methods & Clinical Development;2024-09

2. Potential Role of Hydrogels in Stem Cell Culture and Hepatocyte Differentiation;Nano Biomedicine and Engineering;2024-06

3. A comprehensive review on nanocomposite biomaterials based on gelatin for bone tissue engineering;International Journal of Biological Macromolecules;2024-01

4. The adaptation of bovine embryonic stem cells to the changes of feeder layers;In Vitro Cellular & Developmental Biology - Animal;2023-02

5. Xeno-free culture and proliferation of hPSCs on 2D biomaterials;Progress in Molecular Biology and Translational Science;2023

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