Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling-Reference-Cited by-同舟云学术

Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling

Published:2017 Issue:12 Volume:142 Page:2239-2246
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Jung Se-Hui¹²³⁴,Kong Deok-Hoon¹²³⁴,Jeon Hye-Yoon¹²³⁴,Han Eun-Taek⁵²³⁴,Park Won Sun⁶²³⁴,Hong Seok-Ho⁷²³⁴,Kim Young-Myeong¹²³⁴,Ha Kwon-Soo¹²³⁴^ORCID

Affiliation:

1. Department of Molecular and Cellular Biochemistry

2. Kangwon National University School of Medicine

3. Kangwon-Do 24341

4. Korea

5. Department of Medical Environmental Biology and Tropical Medicine

6. Department of Physiology

7. Department of Internal Medicine

Abstract

An on-chip protein kinase assay for profiling kinase kinetic parameters by introducing the substrate affinity (K_m) and the phosphorylation rate (V_p) under physiological conditions.

Funder

National Research Foundation of Korea

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AN/C6AN02682F

Reference31 articles.

1. The Protein Kinase Complement of the Human Genome

2. The nuts and bolts of AGC protein kinases

3. Protein kinases: evolution of dynamic regulatory proteins

4. Regulation and Function of Protein Kinases and Phosphatases

5. Global analysis of protein phosphorylation in yeast

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