ApoHRP-based assay to measure intracellular regulatory heme
Author:
Affiliation:
1. Department of Basic Sciences
2. TCMC
3. Scranton, USA
4. Department of Medical Education
5. California Northstate University (CNSU)
6. Department of Biochemistry
7. University of California at Riverside
8. Riverside, USA
Abstract
Apo-peroxidase requires heme to properly fold into the holoperoxidase. ‘Free’ (or uncommitted) heme is transferred quickly to apo-peroxidase to form the active enzyme; heme from heme-proteins that binds heme with low affinity (or exchangeable) is very slow to transfer; while heme from stable heme-proteins does not transfer.
Funder
American Federation for Aging Research
Foundation for the National Institutes of Health
Publisher
Oxford University Press (OUP)
Subject
Metals and Alloys,Biochemistry,Biomaterials,Biophysics,Chemistry (miscellaneous)
Link
http://pubs.rsc.org/en/content/articlepdf/2015/MT/C4MT00246F
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