Large scale microfluidic CRISPR screening for increased amylase secretion in yeast-Reference-Cited by-同舟云学术

Large scale microfluidic CRISPR screening for increased amylase secretion in yeast

Published:2023 Issue:16 Volume:23 Page:3704-3715
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Johansson S. Andreas¹^ORCID,Dulermo Thierry²,Jann Cosimo¹,Smith Justin D.³⁴^ORCID,Pryszlak Anna¹,Pignede Georges²^ORCID,Schraivogel Daniel¹^ORCID,Colavizza Didier²,Desfougères Thomas²,Rave Christophe²,Farwick Alexander²^ORCID,Merten Christoph A.¹^ORCID,Roy Kevin R.³⁴,Wei Wu³⁴,Steinmetz Lars M.¹³⁴^ORCID

Affiliation:

1. European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Heidelberg, Germany

2. Lesaffre Institute of Science & Technology, Lesaffre, 59700 Marcq-en-Baroeul, France

3. Department of Genetics, Stanford University School of Medicine, Stanford, California, USA

4. Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA

Abstract

Large scale perturbation of gene expression in yeast using CRISPR libraries, coupled with high-throughput screening using fluorescence-based sorting of microfluidic droplets, to identify genes important for increased α-amylase secretion.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2023/LC/D3LC00111C

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