Coomassie Brilliant Blue-binding: a simple and effective method for the determination of water-insoluble protein surface hydrophobicity

Author:

Cao Yungang12345,Zhao Jing6789,Xiong Youling L.6789

Affiliation:

1. State Key Laboratory of Food Science and Technology

2. Synergetic Innovation Center of Food Safety and Nutrition

3. School of Food Science and Technology

4. Jiangnan University

5. Wuxi

6. Department of Animal and Food Sciences

7. University of Kentucky

8. Lexington

9. USA

Abstract

A novel Coomassie Brilliant Blue-binding method, which correlated well with the widely accepted ANS fluorescence method (R = 0.95), was developed to determine the surface hydrophobicity of water-insoluble proteins.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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