Direct chemiluminescence detection of circulating microRNAs in serum samples using a single-strand specific nuclease-distinguishing nucleic acid hybrid system-Reference-Cited by-同舟云学术

Direct chemiluminescence detection of circulating microRNAs in serum samples using a single-strand specific nuclease-distinguishing nucleic acid hybrid system

Published:2018 Issue:15 Volume:54 Page:1909-1912
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Ling Kai¹²³⁴⁵^ORCID,Jiang Hongyan⁶⁷⁸⁵,Huang Xue¹²³⁴⁵,Li Yang¹²³⁴⁵,Lin Juanjuan¹²³⁴⁵,Li Fu-Rong¹²³⁴⁵

Affiliation:

1. Translational Medicine Collaborative Innovation Center

2. The Second Clinical Medical College (Shenzhen People's Hospital)

3. Jinan University

4. Shenzhen 518020

5. China

6. Department of Pharmacy

7. Shantou University Medical College

8. Shantou 515041

Abstract

We developed a microplate-based enhanced chemiluminescence system for the direct detection of circulating miRNAs. The system exhibited a high target sensitivity and specificity, with a detection limit of 3.02 fM.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Guangdong Province

Medical Science and Technology Foundation of Guangdong Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2018/CC/C7CC09087K

Reference30 articles.

1. MicroRNAs

2. MicroRNAs in Cancer

3. Circulating MicroRNA Biomarker Studies: Pitfalls and Potential Solutions

4. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases

5. MicroRNA: Function, Detection, and Bioanalysis

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