Benchmarking classification abilities of novel optical photothermal IR spectroscopy at the single-cell level with bulk FTIR measurements

Author:

Richardson Paul I. C.1,Horsburgh Malcolm J.2,Goodacre Royston1ORCID

Affiliation:

1. Centre for Metabolomics Research, Department of Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, BioSciences Building, Crown St, Liverpool, UK

2. Microbiology Research Group, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, BioSciences Building, Crown St, Liverpool, UK

Abstract

Optical photothermal IR spectroscopy (O-PTIR) presents new opportunities for single-bacterium analysis. Here, we assess its ability to classify bacteria at various levels of similarity.

Funder

Engineering and Physical Sciences Research Council

Natural Environment Research Council

University of Liverpool

Medical Research Council

Publisher

Royal Society of Chemistry (RSC)

Reference33 articles.

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4. Single-cell analysis using Fourier transform infrared microspectroscopy

5. Bruker , Research FT-IR and QCL Microscope HYPERION II , https://www.bruker.com/en/products-and-solutions/infrared-and-raman/ft-ir-microscopes/hyperion-ii-ft-ir-and-qcl-microscope.html , accessed 6 March 2024

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