Fully integrated on-line strategy for highly sensitive proteome profiling of 10–500 mammalian cells

Author:

Yang Yun123,Sun Suhong4,He Shunji4,Liu Chengmin5ORCID,Fu Changying1,Tang Min6,Liu Chao6,Sun Ying5,Lam Henry2,Liu Zhiyong4,Tian Ruijun1ORCID

Affiliation:

1. Department of Chemistry, School of Science, Southern University of Science and Technology, No. 1088 Xueyuan Avenue, Nanshan District, Shenzhen 518055, China

2. Department of Chemical and Biological Engineering, The Hong Kong University of Science &Technology, Clear Water Bay, Kowloon, Hong Kong

3. South China Institute of Biomedicine, No. 83 Ruihe Road, GuangZhou 510535, China

4. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China

5. Department of Biology, Southern University of Science and Technology, No. 1088 Xueyuan Avenue, Nanshan District, Shenzhen 518055, China

6. Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, Beihang University and Key Laboratory of Big Data-Based Precision Medicine (Beihang University), Ministry of Industry and Information Technology, Beijing, 100191, China

Abstract

3-frit mixed-mode RCPR enabled highly sensitive proteome profiling of 10–500 mammalian cells and provided an alternative to nanofluidic devices.

Funder

National Key Basic Research Program For Youth

National Science Fund for Distinguished Young Scholars

Southern University of Science and Technology

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling;Methods in Molecular Biology;2024

2. Bottom-Up Proteomics: Advancements in Sample Preparation;International Journal of Molecular Sciences;2023-03-10

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