Role of E270 in pH- and metal-sensitivities of firefly luciferases-Reference-Cited by-同舟云学术

Role of E270 in pH- and metal-sensitivities of firefly luciferases

Published:2020 Issue:11 Volume:19 Page:1548-1558
ISSN:1474-905X
Container-title:Photochemical & Photobiological Sciences
language:en
Short-container-title:Photochem. Photobiol. Sci.

Author:

Viviani V. R.¹²³⁴⁵^ORCID,Pelentir G. F.¹²³⁴⁵,Oliveira G.⁶²⁷⁵^ORCID,Tomazini A.⁸⁹¹⁰⁵,Bevilaqua V. R.⁶²⁷⁵

Affiliation:

1. Graduate Program of Biotechnology and Environmental Monitoring

2. Federal University of São Carlos (UFSCar)

3. Rodovia João Leme dos Santos

4. Sorocaba

5. Brazil

6. Graduate Program of Evolutive Genetics and Molecular Biology

7. São Carlos

8. Brazilian Biorenewables Energy National Laboratory (LNBR)

9. Brazilian Center for Research in Energy and Materials (CNPEM)

10. Campinas

Abstract

The substitutions of the conserved residue E270 at the N-terminal of α-helix 10 in pH-sensitive firefly luciferases stabilize a green emitting active site conformation, decreasing pH-sensitivity.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Springer Science and Business Media LLC

Subject

Physical and Theoretical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/PP/D0PP00190B

Reference49 articles.

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3. BIOLUMINESCENCE OF BRAZILIAN FIREFLIES (COLEOPTERA: LAMPYRIDAE): SPECTRAL DISTRIBUTION and pH EFFECT ON LUCIFERASE-ELICITED COLORS. COMPARISON WITH ELATERID and PHENGODID LUCIFERASES

4. Biotechnological applications of bioluminescence and chemiluminescence

5. V. R. Viviani and Y.Ohmiya , Beetle luciferases: Colorful lights on biological processes and diseases of referencing Photoproteins in Bioanalysis , ed. S. Daunert and S. K. Deo , Wiley , New York , 2006 , vol. 3 , pp. 49–60

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