A modified SELEX approach to identify DNA aptamers with binding specificity to the major histocompatibility complex presenting ovalbumin model antigen-Reference-Cited by-同舟云学术

A modified SELEX approach to identify DNA aptamers with binding specificity to the major histocompatibility complex presenting ovalbumin model antigen

Published:2023 Issue:46 Volume:13 Page:32681-32693
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Lin Yang¹^ORCID,Chen Cho-Yi²^ORCID,Ku Yu-Chia²,Wang Li-Chin²,Hung Chia-Chien³,Lin Zhi-Qian¹,Chen Bing-Hong¹,Hung Jui-Tse⁴,Sun Yi-Chen⁵⁶,Hung Kai-Feng¹⁷^ORCID

Affiliation:

1. Department of Medical Research, Taipei Veterans General Hospital, 201, Section 2, Shi-Pai Road, Taipei 112, Taiwan

2. Institute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei, Taiwan

3. School of Computer Science, Georgia Institute of Technology Atlanta, GA, USA

4. Kang Chiao International School, Taipei, Taiwan

5. School of Medicine, Tzu-Chi University, Hualien, Taiwan

6. Department of Ophthalmology, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan

7. Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan

Abstract

A modified SELEX that employs consecutive selections with minimal PCR and selects aptamers that were identified in multiple selection rounds enables the identification of aptamers with binding affinity to specific pMHC complex.

Funder

National Science and Technology Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/RA/D3RA04686A

Reference62 articles.

1. In vitro selection of RNA molecules that bind specific ligands

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5. The Chemical Biology of Aptamers

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