Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery-Reference-Cited by-同舟云学术

Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery

Published:2020 Issue:12 Volume:20 Page:2062-2074
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Brower Kara K.¹²³⁴⁵,Carswell-Crumpton Catherine⁶²³⁴,Klemm Sandy⁷²³⁴,Cruz Bianca⁸⁹¹⁰⁴,Kim Gaeun¹²³⁴,Calhoun Suzanne G. K.¹¹²³⁴,Nichols Lisa⁶²³⁴,Fordyce Polly M.¹²³⁴⁵^ORCID

Affiliation:

1. Department of Bioengineering

2. Stanford University

3. Stanford

4. USA

5. Chem-H Institute

6. Center for Molecular and Genetic Medicine

7. Department of Genetics

8. Department of Physics

9. California State Polytechnic Institute

10. Pomona

11. Department of Chemical Engineering

Abstract

We have developed a novel workflow (sdDE-FACS, s̲ingle d̲roplet D̲ouble E̲mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.

Funder

NIH Office of the Director

National Institutes of Health

National Science Foundation

Thomas and Stacey Siebel Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/D0LC00261E

Reference63 articles.