Deep eutectic solvents as a stabilising medium for NAD coenzyme: unravelling the mechanism behind coenzyme stabilisation effect

Author:

Radović Mia1ORCID,Hok Lucija2ORCID,Panić Manuela1,Cvjetko Bubalo Marina1ORCID,Vianello Robert2ORCID,Vinković Marijana2,Radojčić Redovniković Ivana1

Affiliation:

1. University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia

2. Ruđer Bošković Institute, Zagreb, Croatia

Abstract

DES choline chloride:urea effectively stabilises NAD coenzyme up to 50 days at room temperature. Consequently, this solvent could be used to minimise NAD degradation during storage, assays, and enzyme-catalysed oxidoreductive reactions.

Funder

Hrvatska Zaklada za Znanost

European Regional Development Fund

Publisher

Royal Society of Chemistry (RSC)

Subject

Pollution,Environmental Chemistry

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