<i>Streptomyces</i> cell-free systems for natural product discovery and engineering-Reference-Cited by-同舟云学术

Streptomyces cell-free systems for natural product discovery and engineering

Published:2023 Issue:2 Volume:40 Page:228-236
ISSN:0265-0568
Container-title:Natural Product Reports
language:en
Short-container-title:Nat. Prod. Rep.

Author:

Moore Simon J.¹²^ORCID,Lai Hung-En³^ORCID,Li Jian⁴^ORCID,Freemont Paul S.⁵

Affiliation:

1. School of Biosciences, University of Kent, UK

2. School of Biological and Behavioural Sciences, Queen Mary University of London, UK

3. School of Biological Sciences, Victoria University of Wellington, New Zealand

4. School of Physical Science and Technology, ShanghaiTech University, China

5. Department of Medicine, Imperial College London, UK

Abstract

Streptomyces cell-free gene expression (CFE) systems for natural product discovery. The CFE system simply requires DNA, a cell-extract, amino acids, and a metabolite solution to study transcription, translation, and biosynthesis in a single reaction.

Funder

Royal Society

Wellcome Trust

Engineering and Physical Sciences Research Council

Leverhulme Trust

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Drug Discovery,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/NP/D2NP00057A