Liquid chromatography mass spectrometry based characterization of epitope configurations

Author:

Levernæs Maren Christin Stillesby1234,Moe Arelí Urtubia1234,Bøe Sigurd Leinæs56784,Paus Elisabeth56784,Reubsaet Léon1234ORCID,Halvorsen Trine Grønhaug1234ORCID

Affiliation:

1. Department of Pharmacy

2. University of Oslo

3. 0316 Oslo

4. Norway

5. Department of Medical Biochemistry

6. Norwegian Radium Hospital

7. Oslo University Hospital

8. Oslo

Abstract

Here we evaluate a quick and easy tool for determination of epitope configuration using immunocapture and liquid chromatography mass spectrometry (LC-MS) subsequent to pre-treatment of the target protein to disrupt its three-dimensional structure.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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