Covalently attached intercalators restore duplex stability and splice-switching activity to triazole-modified oligonucleotides-Reference-Cited by-同舟云学术

Covalently attached intercalators restore duplex stability and splice-switching activity to triazole-modified oligonucleotides

Published:2022 Issue:6 Volume:3 Page:765-772
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Dysko Anna¹,Baker Ysobel R.¹^ORCID,McClorey Graham²,Wood Matthew J. A.²,Fenner Sabine³,Williams Glynn³,El-Sagheer Afaf¹⁴,Brown Tom¹^ORCID

Affiliation:

1. Department of Chemistry, Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK

2. Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK

3. GSK Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK

4. Chemistry Branch Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt

Abstract

Oligonucleotides can bind to mRNA and alter gene expression, but require backbone modifications for clinical use. We show that attaching pyrene or anthraquinone to the ribose sugar next to an artificial triazole backbone restores duplex stability and splice switching ability in cells.

Funder

Biotechnology and Biological Sciences Research Council

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CB/D2CB00100D

Reference47 articles.

1. Therapeutic Oligonucleotides: State of the Art

2. The chemical evolution of oligonucleotide therapies of clinical utility

3. Antisense technology: A review

4. Molecular Mechanisms of Antisense Oligonucleotides

5. RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus

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