Mutasynthesis generates nine new pyrroindomycins

Author:

Wu Zhuhua12,Xia Zhengxiang23ORCID,Tang Zhijun2ORCID,Li Ji'an1,Liu Wen2

Affiliation:

1. National key Laboratory of Lead druggability Research, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, 285 Copernicus Road, Shanghai 201203, China

2. State Key Laboratory of Chemical Biology, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China

3. Department of Pharmacy, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 399 Middle Yan Chang Road, Shanghai, 200072, China

Abstract

Nine new pyrroindomycins with diverse acyl modification on their deoxy-trisaccharide moieties were created via a mutasynthesis approach. The key feature lies in blocking DHPI formation by gene pyrK1 inactivation and supplying chemical acyl precursors.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Publisher

Royal Society of Chemistry (RSC)

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