Observation of liquid–liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy-Reference-Cited by-同舟云学术

Observation of liquid–liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy

Published:2021 Issue: Volume: Page:
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Murakami Kazuki¹²³⁴^ORCID,Kajimoto Shinji¹²³⁴^ORCID,Shibata Daiki¹²³⁴^ORCID,Kuroi Kunisato⁵⁶⁷⁴^ORCID,Fujii Fumihiko⁵⁶⁷⁴^ORCID,Nakabayashi Takakazu¹²³⁴^ORCID

Affiliation:

1. Graduate School of Pharmaceutical Sciences

2. Tohoku University

3. Sendai 980-8578

4. Japan

5. Faculty of Pharmaceutical Sciences

6. Kobe Gakuin University

7. Kobe 650-8586

Abstract

Liquid–liquid phase separation (LLPS) plays an important role in a variety of biological processes. We have established a method to quantify a single droplet formed by LLPS using the Raman band of water as an internal standard.

Funder

Japan Society for the Promotion of Science

Sumitomo Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC06095J

Reference57 articles.

1. Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

2. Valence and patterning of aromatic residues determine the phase behavior of prion-like domains