Experimental and theoretical approaches for the selective detection of thymine in real samples using gold nanoparticles as a biochemical sensor

Author:

Shrivas Kamlesh12345ORCID,Nirmalkar Nidhi5674,Thakur Santosh Singh5674,Kurrey Ramsingh1234,Sinha Deepak5834,Shankar Ravi9101112

Affiliation:

1. School of Studies in Chemistry

2. Pt. Ravishankar Shukla University

3. Raipur

4. India

5. Department of Chemistry

6. Guru Ghasidas Vishwavidyalaya

7. Bilaspur

8. Government Nagarjuna Post Graduate College of Science

9. Nanoscience and Nanoengineering Program

10. South Dakota School of Mines and Technology

11. Rapid City

12. USA

Abstract

We report a simple, selective and cost effective method for the qualitative and quantitative determination of thymine in a DNA standard and in urine samples using gold nanoparticles (AuNPs) as a label-free colorimetric biochemical sensor.

Funder

Science and Engineering Research Board

Department of Science and Technology, Ministry of Science and Technology

University Grants Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Reference39 articles.

1. J. M.Berg , J. L.Tymoczko and L.Stryer , Biochemistry , WH Freeman and Company , 5 th edn, 2002 , pp. 118–119

2. B.Alberts , A.Johnson , J.Lewis , M.Raff , K.Roberts and P.Walter , Molecular Biology of the Cell , 6 th edn, 2014 , ch. 4, DNA, Chromosomes and Genomes, Garland Science, pp. 1–1464

3. Determination of purine bases by reversed-phase high-performance liquid chromatography using real-time surface-enhanced Raman spectroscopy

4. Electrochemical behavior of anticancer drug 5-fluorouracil at carbon paste electrode and its analytical application

5. High-performance liquid chromatographic analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma

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