Re-assessment of monoclonal antibodies against diclofenac for their application in the analysis of environmental waters

Author:

Schmidt Stephan12,Hoffmann Holger13,Garbe Leif-Alexander4,Harrer Andrea5,Steiner Markus5,Himly Martin5ORCID,Schneider Rudolf J.12ORCID

Affiliation:

1. Department of Analytical Chemistry, Reference Materials, BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, 12489 Berlin, Germany

2. Technische Universität Berlin, Straße des 17. Juni 135, D-10623 Berlin, Germany

3. Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, D-12489 Berlin, Germany

4. Hochschule Neubrandenburg, Fachbereich Agrarwirtschaft und Lebensmittelwissenschaften, D-17033 Neubrandenburg, Germany

5. Department of Biosciences and Medical Biology, Division of Allergy and Immunology, Paris Lodron University of Salzburg, A-5020 Salzburg, Austria

Abstract

A formerly developed antibody against diclofenac was re-assessed for its application in an indirect competitive ELISA to determine the pharmaceutical in wastewater. A series of matrix influences and cross-reactivities have been investigated.

Funder

Austrian Science Fund

Publisher

Royal Society of Chemistry (RSC)

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