A concentration-descending washing strategy with methanol for the enhancement of protein imaging in biological tissues by MALDI-MS-Reference-Cited by-同舟云学术

A concentration-descending washing strategy with methanol for the enhancement of protein imaging in biological tissues by MALDI-MS

Published:2023 Issue:4 Volume:148 Page:823-831
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Xu Hualei¹²,Hao Qichen¹²,Liu Haiqiang¹²,Chen Lulu¹²^ORCID,Wu Ran¹²,Qin Liang¹²,Guo Hua¹²,Li Jinrong¹²,Yang Chenyu¹²,Hu Hao¹²,Xue Kun²,Feng Jinchao²,Zhou Yijun²,Liu Biao³,Li Gaopeng⁴,Wang Xiaodong¹²^ORCID

Affiliation:

1. Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Beijing 100081, China

2. College of Life and Environmental Sciences, Centre for Imaging & Systems Biology, Minzu University of China, Beijing 100081, China

3. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment of China, Nanjing 210042, China

4. General Surgery Department, Shanxi Bethune Hospital, Taiyuan 030032, China

Abstract

A concentration-descending washing strategy with methanol improves the performance of MALDI-MSI for in situ protein detection.

Funder

Minzu University of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D2AN01678H

Reference34 articles.

1. Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution

2. Automated annotation and visualisation of high-resolution spatial proteomic mass spectrometry imaging data using HIT-MAP

3. In situ molecular imaging of proteins in tissues using mass spectrometry

4. Proteomics: Technologies and Their Applications

5. Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis coupled with mass spectrometry

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