Fluorescent molecular rotors detect <i>O</i><sup>6</sup>-methylguanine dynamics and repair in duplex DNA-Reference-Cited by-同舟云学术

Fluorescent molecular rotors detect O⁶-methylguanine dynamics and repair in duplex DNA

Published:2024 Issue:9 Volume:60 Page:1156-1159
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Copp William¹²,Karimi Ashkan¹²,Yang Tianxiao²³,Guarné Alba²³,Luedtke Nathan W.¹²⁴^ORCID

Affiliation:

1. Department of Chemistry, McGill University, H3A-0B8 Montreal, Canada

2. Centre de Recherche en Biologie Structural, McGill University, H3G 0B1 Montreal, Canada

3. Department of Biochemistry, McGill University, H3G 1Y6 Montreal, Canada

4. Department of Pharmacology and Therapeutics, McGill University, H3A-1A3 Montreal, Canada

Abstract

Alkylation at the O6 position of guanine is a common and highly mutagenic form of DNA damage. Here, dynamic changes in fluorescence from a molecular rotor base analogue are used to track O6 alkylation and repair in duplex DNA.

Funder

Natural Sciences and Engineering Research Council of Canada

Fonds de recherche du Québec – Nature et technologies

Canada Foundation for Innovation

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CC/D3CC04782B

Reference52 articles.

1. Direct Reversal of DNA Alkylation Damage

2. Nonenzymatic methylation of DNA by the intracellular methyl group donor S-adenosyl-L-methionine is a potentially mutagenic reaction.

3. Multifaceted Roles of Alkyltransferase and Related Proteins in DNA Repair, DNA Damage, Resistance to Chemotherapy, and Research Tools

4. Ubiquitination-Dependent Proteolysis of O6-Methylguanine-DNA Methyltransferase in Human and Murine Tumor Cells following Inactivation with O6-Benzylguanine or 1,3-Bis(2-chloroethyl)-1-nitrosourea