A mechanistic study on the cellular uptake, intracellular trafficking, and antisense gene regulation of bottlebrush polymer-conjugated oligonucleotides-Reference-Cited by-同舟云学术

A mechanistic study on the cellular uptake, intracellular trafficking, and antisense gene regulation of bottlebrush polymer-conjugated oligonucleotides

Published:2023 Issue:2 Volume:4 Page:138-145
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Zhang Lei¹²,Wang Yuyan²,Chen Peiru²,Wang Dali²,Sun Tingyu²,Zhang Zheyu²,Wang Ruimeng²,Kang Xi²^ORCID,Fang Yang²,Lu Hao²,Cai Jiansong²,Ren Mengqi²,Dong Sijia S.²^ORCID,Zhang Ke²³^ORCID

Affiliation:

1. Chemicobiology and Functional Materials Institute, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China

2. Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA

3. Departments of Chemical Engineering and Bioengineering, Northeastern University, Boston, Massachusetts 02115, USA

Abstract

We show that a bottlebrush polymer-antisense oligonucleotide conjugate (termed pacDNA) enters cancer cells by scavenger receptor-mediated endocytosis and micropinocytosis, trafficks via the endolysosomal pathway, and functions as a steric blocker.

Funder

National Cancer Institute

National Institute of General Medical Sciences

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2023/CB/D2CB00149G

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