Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction-Reference-Cited by-同舟云学术

Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction

Published:2018 Issue:4 Volume:6 Page:863-876
ISSN:2047-4830
Container-title:Biomaterials Science
language:en
Short-container-title:Biomater. Sci.

Author:

VerHeul Ross¹²³⁴⁵,Sweet Craig¹²³⁴⁵,Thompson David H.¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemistry

2. Purdue Center for Cancer Research

3. Multi-disciplinary Cancer Research Facility

4. Purdue University

5. West Lafayette

Abstract

Elastin-like peptide (ELP) constructs are typically hydrophobic in nature. A rapid organic solvent extraction method is reported that affords highly pure ELP with removal of key contaminants such as nucleic acids and LPS.

Funder

National Cancer Institute

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering

Link

http://pubs.rsc.org/en/content/articlepdf/2018/BM/C8BM00124C

Reference63 articles.

1. Sequence Variation of Bovine Elastin mRNA Due to Alternative Splicing

2. Free energy transduction in polypeptides and proteins based on inverse temperature transitions

3. Physical Chemistry of Biological Free Energy Transduction As Demonstrated by Elastic Protein-Based Polymers

4. Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity

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