Gel-seq: whole-genome and transcriptome sequencing by simultaneous low-input DNA and RNA library preparation using semi-permeable hydrogel barriers-Reference-Cited by-同舟云学术

Gel-seq: whole-genome and transcriptome sequencing by simultaneous low-input DNA and RNA library preparation using semi-permeable hydrogel barriers

Published:2017 Issue:15 Volume:17 Page:2619-2630
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Hoople Gordon D.¹²³⁴⁵^ORCID,Richards Andrew⁶⁷⁸⁴^ORCID,Wu Yan⁶⁷⁸⁴,Kaneko Kota⁹¹⁰⁷⁸⁴,Luo Xiaolin⁹¹⁰⁷⁸⁴,Feng Gen-Sheng⁹¹⁰⁷⁸⁴,Zhang Kun⁶⁷⁸⁴,Pisano Albert P.⁵⁷⁸⁴

Affiliation:

1. Shiley-Marcos School of Engineering

2. University of San Diego

3. San Diego

4. USA

5. Department of Mechanical and Aerospace Engineering

6. Department of Bioengineering

7. University of California San Diego

8. La Jolla

9. Department of Pathology

10. School of Medicine

Abstract

Gel-seq enables researchers to simultaneously prepare libraries for both DNA and RNA from 100 cells.

Funder

National Science Foundation

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2017/LC/C7LC00430C

Reference27 articles.

1. Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells

2. Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity

3. Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

4. Translational and clinical implications of the genetic landscape of prostate cancer

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