Rapid time-lapse 3D oxygen tension measurements within hydrogels using widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) and image segmentation

Author:

Chang Dao-Ming1,Hsu Heng-Hua12,Ko Ping-Liang13,Chang Wei-Jen4,Hsieh Tung-Han1,Wu Hsiao-Mei15ORCID,Tung Yi-Chung1ORCID

Affiliation:

1. Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan

2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu 30013, Taiwan

3. Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan

4. Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan

5. Department of Biomechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan

Abstract

An approach integrating widefield frequency-domain fluorescence lifetime imaging microscopy and image segmentation to achieve rapid time-lapse 3D oxygen tension measurements has been developed.

Funder

National Science and Technology Council

Academia Sinica

Publisher

Royal Society of Chemistry (RSC)

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