Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples-Reference-Cited by-同舟云学术

Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples

Published:2019 Issue:44 Volume:11 Page:5730-5735
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Pacocha Natalia¹²³⁴^ORCID,Scheler Ott¹²³⁴⁵,Nowak Mikolaj Marcin⁶⁷⁸⁴,Derzsi Ladislav¹²³⁴^ORCID,Cichy Joanna⁶⁷⁸⁴,Garstecki Piotr¹²³⁴^ORCID

Affiliation:

1. Institute of Physical Chemistry

2. Polish Academy of Sciences

3. 01-224 Warsaw

4. Poland

5. Institute of Molecular and Cell Biology

6. Department of Immunology

7. Jagiellonian University

8. 30-387 Cracow

Abstract

Here we demonstrate a direct droplet digital PCR assay (dddPCR) for simultaneous identification and quantification of bacteria in a bacterial mixture.

Funder

Fundacja na rzecz Nauki Polskiej

Eesti Teadusagentuur

Narodowe Centrum Nauki

Tallinna Tehnikaülikool

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AY/C9AY01874C

Reference36 articles.

1. Comparison of three rapid and easy bacterial DNA extraction methods for use with quantitative real-time PCR

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