A single filament biomechanical study of the enteropathogenic Escherichia coli Type III secretion system reveals a high elastic aspect ratio

Author:

Elias-Mordechai Moran1,David Nofar2,Oren Sonia2,Georgia Pelah Maya1,Jopp Jürgen3,Fichtman Boris4,Harel Amnon4ORCID,Berkovich Ronen13ORCID,Sal-Man Neta2ORCID

Affiliation:

1. Department of Chemical Engineering, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel

2. Department of Microbiology, Immunology, and Genetics, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel

3. The Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel

4. Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel

Abstract

The mechanical response of individual T3SS filaments was measured by pulling on filaments extending out from bacterial surfaces and by pressing into detached filaments. The longitudinal and radial elastic moduli yielded an aspect ratio of ∼1 : 220.

Funder

Israel Science Foundation

Ben-Gurion University of the Negev

Ministry of Science and Technology, Israel

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

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