Universal probe-based SNP genotyping with visual readout: a robust and versatile method

Author:

Wang Zhongzhong1,Zhang Zhang1ORCID,Luo Wang1,Wang Luojia1,Han Xiaole1,Zhao Rong1,Liu Xin1,Zhang Jianhong2,Yu Wen3,Li Junjie1,Yang Yujun1,Zuo Chen1,Xie Guoming1ORCID

Affiliation:

1. Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical Laboratory Microfluidics and SPRi Engineering Research Center, Chongqing Medical University, Chongqing 400016, P.R. China

2. Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, P.R. China

3. Chongqing University Cancer Hospital and Chongqing Cancer Institute, Chongqing 400030, P.R. China

Abstract

A universal “typing formula” for SNP genotyping was established by introducing a tail at the 5′ end of an allele primer, which was matched with a small molecule probe and combined with a lateral flow assay.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

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