Extraction of an active enzyme by self-buffering ionic liquids: a green medium for enzymatic research-Reference-Cited by-同舟云学术

Extraction of an active enzyme by self-buffering ionic liquids: a green medium for enzymatic research

Published:2016 Issue:22 Volume:6 Page:18567-18576
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Gupta Bhupender S.¹²³⁴,Taha Mohamed¹²³⁴⁵,Lee Ming-Jer¹²³⁴

Affiliation:

1. Department of Chemical Engineering

2. National Taiwan University of Science and Technology

3. Taipei 106-07

4. Taiwan

5. Departamento de Química

Abstract

The extraction of a model enzyme α-chymotrypsin in its active conformation from an aqueous solution by using new biocompatible and self-buffering Good's buffer ionic liquids-based aqueous biphasic systemsis demonstrated.

Funder

Ministry of Science and Technology, Taiwan

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/RA/C6RA00607H

Reference83 articles.

1. Overview of the Stability of α-Chymotrypsin in Different Solvent Media

2. Reversible folding–unfolding, aggregation protection, and multi-year stabilization, in high concentration protein solutions, using ionic liquids

3. Formation and dissolution of hen egg white lysozyme amyloid fibrils in protic ionic liquids

4. How ionic liquids can help to stabilize native proteins

5. Patterns of protein unfolding and protein aggregation in ionic liquids

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