Design of a protein tag and fluorogenic probe with modular structure for live-cell imaging of intracellular proteins-Reference-Cited by-同舟云学术

Design of a protein tag and fluorogenic probe with modular structure for live-cell imaging of intracellular proteins

Published:2016 Issue:1 Volume:7 Page:308-314
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Kamikawa Yuko¹²³⁴,Hori Yuichiro⁵²³⁴¹,Yamashita Kazuo¹²³⁴,Jin Lin¹²³⁴,Hirayama Shinya⁵²³⁴,Standley Daron M.¹²³⁴,Kikuchi Kazuya⁵²³⁴¹

Affiliation:

1. IFReC

2. Osaka University

3. Osaka 565-0871

4. Japan

5. Graduate School of Engineering

Abstract

Quick and no-wash labeling of intracellular proteins was achieved in live cells using a PYP-tag mutant and a membrane-permeable fluorogenic probe with modular structures.

Funder

Ministry of Education, Culture, Sports, Science, and Technology

Core Research for Evolutional Science and Technology, Japan Science and Technology Agency

Asahi Glass Foundation

Japan Science and Technology Agency

Mochida Memorial Foundation for Medical and Pharmaceutical Research

Naito Foundation

Uehara Memorial Foundation

Japan Society for the Promotion of Science

Osaka University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/SC/C5SC02351C

Reference40 articles.

1. Chemical Tags for Labeling Proteins Inside Living Cells

2. Small-Molecule-Based Protein-Labeling Technology in Live Cell Studies: Probe-Design Concepts and Applications

3. Fluorescence and bioluminescence procedures for functional proteomics

4. Combinatorial Strategies in Fluorescent Probe Development

5. Genetically encoded biosensors based on engineered fluorescent proteins

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