Circulating microRNA detection using electrochemical biosensor for rapid diagnosis of liver disease in dogs

Author:

Roychoudhury Appan1ORCID,Diez Federico2,Mellanby Richard J.2,Dear James W.3ORCID,Bachmann Till T.1ORCID

Affiliation:

1. Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, 4-5 Little France Drive, Edinburgh EH16 4UU, UK

2. The Royal (Dick) School of Veterinary Studies and Roslin Institute, The Hospital for Small Animals, University of Edinburgh, Easter Bush Campus, Edinburgh EH25 9RG, UK

3. Centre for Cardiovascular Science, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK

Abstract

We report a new combination of short probe and target overhangs during hybridisation with a flow-based sample cycling setup for sensitive and specific detection of microRNA-122 biomarker. This setup was used towards liver injury diagnostics in dogs.

Funder

Rosetrees Trust

Publisher

Royal Society of Chemistry (RSC)

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