Force determination in lateral magnetic tweezers combined with TIRF microscopy-Reference-Cited by-同舟云学术

Force determination in lateral magnetic tweezers combined with TIRF microscopy

Published:2018 Issue:9 Volume:10 Page:4579-4590
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Madariaga-Marcos J.¹²³⁴⁵,Hormeño S.¹²³⁴⁵^ORCID,Pastrana C. L.¹²³⁴⁵,Fisher G. L. M.⁶⁷⁸⁹¹⁰,Dillingham M. S.⁶⁷⁸⁹¹⁰^ORCID,Moreno-Herrero F.¹²³⁴⁵^ORCID

Affiliation:

1. Department of Macromolecular Structures

2. Centro Nacional de Biotecnología

3. Consejo Superior de Investigaciones Científicas

4. 28049 Cantoblanco

5. Spain

6. DNA:Protein Interactions Unit

7. School of Biochemistry

8. Biomedical Sciences Building

9. University of Bristol

10. Bristol

Abstract

We have designed and calibrated a magnetic tweezers module to laterally stretch DNA molecules at a constant force, which can be incorporated into conventional magnetic tweezers. We demonstrate the combination of lateral magnetic tweezers with TIRF microscopy by characterizing DNA binding by ParB.

Funder

H2020 European Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2018/NR/C7NR07344E

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