Immuno-capture of extracellular vesicles for individual multi-modal characterization using AFM, SEM and Raman spectroscopy-Reference-Cited by-同舟云学术

Immuno-capture of extracellular vesicles for individual multi-modal characterization using AFM, SEM and Raman spectroscopy

Published:2019 Issue:15 Volume:19 Page:2526-2536
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Beekman Pepijn¹²³⁴⁵^ORCID,Enciso-Martinez Agustin⁶⁷⁸³^ORCID,Rho Hoon Suk⁴⁵⁸³⁹^ORCID,Pujari Sidharam Pundlik¹²³^ORCID,Lenferink Aufried⁶⁷⁸³,Zuilhof Han¹²³¹⁰¹¹^ORCID,Terstappen Leon W. M. M.⁶⁷⁸³,Otto Cees⁶⁷⁸³^ORCID,Le Gac Séverine⁴⁵⁸³^ORCID

Affiliation:

1. Laboratory of Organic Chemistry

2. Wageningen University

3. The Netherlands

4. Applied Microfluidics for BioEngineering Research

5. MESA+ Institute for Nanotechnology and TechMed Center

6. Medical Cell BioPhysics

7. TechMed Center

8. University of Twente

9. Department of Instructive Biomaterials Engineering

10. School of Pharmaceutical Sciences and Technology

11. Tianjin University

Abstract

Here, we report a platform for the specific capture of tdEVs, followed by their in situ characterization using SEM, Raman spectroscopy and AFM, at the single EV level, in terms of size, size distribution and chemical fingerprinting.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2019/LC/C9LC00081J

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