An efficient pyrrolysyl-tRNA synthetase for economical production of MeHis-containing enzymes

Author:

Hutton Amy E.1ORCID,Foster Jake1,Sanders James E. J.1,Taylor Christopher J.1,Hoffmann Stefan A.1ORCID,Cai Yizhi1,Lovelock Sarah L.1ORCID,Green Anthony P.1ORCID

Affiliation:

1. Manchester Institute of Biotechnology, School of Chemistry, The University of Manchester, Manchester, UK

Abstract

A highly efficient aminoacyl tRNA synthetase (G1PylRSMIFAF) has been developed to produce MeHis-containing proteins. High protein titres can be achieved with low ncAA concentrations (0.1 mM) enabling more economical production of MeHis-containing enzymes.

Funder

European Research Council

UK Research and Innovation

Biotechnology and Biological Sciences Research Council

EPSRC Centre for Doctoral Training in Technology Enhanced Chemical Synthesis

Human Frontier Science Program

Publisher

Royal Society of Chemistry (RSC)

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