Detergent-free isolation of CYP450-reductase's FMN-binding domain in <i>E. coli</i> lipid-nanodiscs using a charge-free polymer-Reference-Cited by-同舟云学术

Detergent-free isolation of CYP450-reductase's FMN-binding domain in E. coli lipid-nanodiscs using a charge-free polymer

Published:2022 Issue:31 Volume:58 Page:4913-4916
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Krishnarjuna Bankala¹^ORCID,Ravula Thirupathi¹^ORCID,Ramamoorthy Ayyalusamy¹^ORCID

Affiliation:

1. Biophysics Program, Department of Chemistry, Macromolecular Science and Engineering, Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109-1055, USA

Abstract

The membrane-anchored flavin mononucleotide binding domain (FBD) of the CYP450 reductase and native lipids were directly extracted from E. coli using charge-free pentyl-inulin polymer.

Funder

National Institute of General Medical Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CC/D1CC07193A

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