Fluorescence of nitrobenzoxadiazole (NBD)-labeled lipids in model membranes is connected not to lipid mobility but to probe location

Author:

Amaro Mariana1234,Filipe Hugo A. L.56789,Prates Ramalho J. P.101112137,Hof Martin1234,Loura Luís M. S.5671415

Affiliation:

1. Department of Biophysical Chemistry

2. J. Heyrovský Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic

3. 182 23 Prague

4. Czech Republic

5. Centro de Química de Coimbra

6. 3004-535 Coimbra

7. Portugal

8. Departamento de Química

9. Faculdade de Ciências e Tecnologia

10. Departamento de Química and Centro de Química de Évora

11. Escola de Ciências e Tecnologia

12. Universidade de Évora

13. 7000-671 Évora

14. Centro de Neurociências e Biologia Celular

15. Universidade de Coimbra

Abstract

By combining the theoretical and experimental approaches, we unveil the true cause of the red-edge excitation shift of NBD probes in membranes (not restricted solvent reorientation, but wide fluorophore distribution instead).

Funder

Czech Science Foundation

Fundação para a Ciência e a Tecnologia

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

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