Phosphorylated 5-ethynyl-2′-deoxyuridine for advanced DNA labeling-Reference-Cited by-同舟云学术

Phosphorylated 5-ethynyl-2′-deoxyuridine for advanced DNA labeling

Published:2015 Issue:15 Volume:13 Page:4589-4595
ISSN:1477-0520
Container-title:Organic & Biomolecular Chemistry
language:en
Short-container-title:Org. Biomol. Chem.

Author:

Seo Siyoong¹²³⁴⁵,Onizuka Kazumitsu¹²³,Nishioka Chieko⁶⁷⁸⁹³,Takahashi Eiki⁶⁷⁸⁹³,Tsuneda Satoshi⁴⁵¹⁰³,Abe Hiroshi¹²³¹¹¹²,Ito Yoshihiro¹²³⁴⁵

Affiliation:

1. Nano Medical Engineering Laboratory

2. Wako

3. Japan

4. Department of Life Science and Medical Bioscience

5. Waseda university

6. Support Unit for Animal Experiment

7. Research Resources Center

8. RIKEN Brain Science Institute

9. Saitama 351-0198

10. Tokyo 162-8480

11. Emergent Bioengineering Materials Research Team

12. RIKEN Center for Emergent Matter Science

Abstract

The representative DNA-labeling agent 5-ethynyl-2′-deoxyuridine (EdU) was chemically modified to improve its function. Monophosphrylation was to enhance its efficiency and bis-pivaloyloxymethyl protecting group was for membrane permeability.

Publisher

Royal Society of Chemistry (RSC)

Subject

Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/OB/C5OB00199D

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