A high-throughput screening method for determining the substrate scope of nitrilases-Reference-Cited by-同舟云学术

A high-throughput screening method for determining the substrate scope of nitrilases

Published:2015 Issue:13 Volume:51 Page:2660-2662
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Black Gary W.¹²³⁴⁵,Brown Nicola L.¹²³⁴⁵,Perry Justin J. B.¹²³⁴⁵,Randall P. David⁶⁷⁸⁹,Turnbull Graeme¹²³⁴⁵,Zhang Meng¹²³⁴⁵

Affiliation:

1. Industrial Biotechnology Research Group

2. Department of Applied Sciences

3. Faculty of Health and Life Sciences

4. Northumbria University

5. Newcastle upon Tyne

6. Chemoxy International Ltd

7. Middlesbrough

8. Cleveland

9. UK

Abstract

We have developed a chromogenic reagent to show nitrilase activity and demonstrate its use with 23 enzymes as cell-free extracts.

Funder

Technology Strategy Board

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2015/CC/C4CC06021K

Reference25 articles.

1. Engineering the third wave of biocatalysis

2. Nitrile hydrolases

3. RETRACTED ARTICLE: The nitrile-degrading enzymes: current status and future prospects

4. Microbial nitrilases: versatile, spiral forming, industrial enzymes

5. Chemoenzymatic production of 1,5-dimethyl-2-piperidone

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