A versatile fluorometric in situ hybridization method for the quantitation of hairpin conformations in DNA self-assembled monolayers-Reference-Cited by-同舟云学术

A versatile fluorometric in situ hybridization method for the quantitation of hairpin conformations in DNA self-assembled monolayers

Published:2020 Issue:13 Volume:145 Page:4522-4531
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

He Jiale¹²³⁴,Hu Xiaochen¹²³⁴,Gao Xiaoyi¹²³⁴,Meng Chenchen¹²³⁴,Li Yunchao¹²³⁴^ORCID,Li Xiaohong¹²³⁴^ORCID,Fan Louzhen¹²³⁴^ORCID,Yu Hua-Zhong⁵⁶⁷⁸^ORCID

Affiliation:

1. College of Chemistry

2. Beijing Normal University

3. Beijing 100875

4. P. R. China

5. Department of Chemistry

6. Simon Fraser University

7. Burnaby

8. Canada

Abstract

We report a versatile fluorometric in-situ hybridization protocol for quantifying hairpin conformations in DNA self-assembled monolayers on substrates, which facilitates the creation of hpDNA-based biosensors with optimal detection performance.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Beijing Municipality

Natural Sciences and Engineering Research Council of Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/D0AN00657B

Reference41 articles.

1. Molecular Beacons: Probes that Fluoresce upon Hybridization

2. Kinetics of conformational fluctuations in DNA hairpin-loops

3. Hybridization kinetics and thermodynamics of molecular beacons

4. Biosensing using hairpin DNA probes

5. Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity

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