Chemically labeled ThUBD permits rapid and super-sensitive imaging of polyubiquitination signals-Reference-Cited by-同舟云学术

Chemically labeled ThUBD permits rapid and super-sensitive imaging of polyubiquitination signals

Published:2022 Issue:15 Volume:147 Page:3434-3443
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Xiao Weidi¹,Huang Shuai¹,Gao Yuan²,Liu Tong¹,Li Naikang³,Sui Xinying⁴,Wang Yonghong⁵,Yang Guan¹,Chang Lei¹,Kang Xianjiang³,Duan Changzhu⁴,Qin Weijie¹^ORCID,Xu Ping¹³⁵⁶⁷,Li Yanchang¹³^ORCID

Affiliation:

1. State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Institute of Lifeomics, Beijing 102206, P. R. China

2. Central Laboratory of College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, P. R. China

3. Hebei Province Key Lab of Research and Application on Microbial Diversity, College of Life Sciences, Hebei University, Baoding, Hebei 071002, P. R. China

4. Department of Cell Biology and Genetics, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016, P. R. China

5. Guizhou University, School of Medicine, Guiyang 550025, P. R. China

6. State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, P. R. China

7. School of Public Health, China Medical University, Shenyang 110122, P. R. China

Abstract

The ThUBD-HRP probe and the consequential developed TUF-WB+ method can detect polyubiquitination signal through one-step incubation with hypersensitivity, unbiased detection and a shorter operation time compared with the antibody method.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Chinese Academy of Medical Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D2AN00577H

Reference25 articles.

1. The Ubiquitin Code

2. Quantifying Ubiquitin Signaling

3. Quantitative Proteomics Reveals the Function of Unconventional Ubiquitin Chains in Proteasomal Degradation

4. Ubiquitin-binding domains — from structures to functions

5. The increasing complexity of the ubiquitin code

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